ABSTRACT
Objective To investigate cell's viability and functions when human hepatocyte cell HepG2 and microcarriers were embedded in collagen gel and cultured in vitro.Methods Ultrasound concussion crushing method was used to get nanofiber microcarrier.HepG2 and microcarriers were embedded in collagen gel and cultured in 12 d.The albumin (ALB),urea and lactate dehydrogenase (LDH) levels in the nutrient solution were measured.Results The cells in nanofiber nanofiber microcarrier/collagen gel group were gathered around the microcarrier and formatted aggregates,the ALB and urea levels increased steadily and reached maximum in day 9,then decreased; In collagen gel without nanofiber microcarrier group cells uniformly distributed,the ALB and urea levels reached maximum in day 3,then decreased rapidly,and on day 6 majority of cells dead.Conclusion Gel entrapped nanofiber microcarriers and hepatoeytes is a high-density and longtime culture method which can possibly be used in the bioartificial liver system.
ABSTRACT
Objective Although chitosan has excellent biocompatibility and biodegradability, it is only soluble in acid solution that is not feasible for application in tissue engineering. The current study is aimed to prepare the carboxymethyl chitosan (CMC) for the purpose of improving the solubility of chitosan to meet the requirement of development of tissue engineering scaffolds. Methods The preparation methods and process conditions, such as the proportion of reactant, reaction temperature, reaction time and so on for three types of carboxymethyl chitosan were studied. Results The results indicated that optimal process conditions of three types of water soluble chitosan were obtained. Conclusion Water solubility of chitosan is greatly improved through carboxymethyl modification, which provides possibilities and foundation for further research.
ABSTRACT
The ever increasing use of the Single-use medical devices(SUD) in clinic has brought safety and convenience to people, while the management of SUD waste also put pressure on the environment. Reprocess and reuse of SUD has become the issue of social concerns. This paper will describe the management manner of FDA for reprocess and reuse of SUD, and then give some suggestion on China's management combining the national conditions of our country.
ABSTRACT
In recent years,implantation medicine and alternative medicine have made great progress.As a resuh.clinical demands of substitutes for damaged organs and tissues have been greatly increased.The artificial organs.the tissue related engineering products,and biomaterials have saved thousands of patient's lives successfully.This article is to prospect for the development of artificial organs,tissue engineering and biomaterials in future vears.It mainlv includes the following aspects:The technical feature,the developing objection,the research direction,as well as a few questions which should be paid attention to in developing the related products.
ABSTRACT
In this study cationic and heparinized polyurethanes (PUs) were synthesized by a two-step solution polymerized method. Cationic and heparinized PUs were investigated by infrared spectroscopy, electron spectroscopy for chemical analysis (ESCA) and turbidity method. At the same time, the PUs proved of good biocompatibility through the laboratory tests, including blood coagulation time (CT), activated partial thromb plastin time (APTT) and fibroblast culture. These materials have good biocompatible function.
Subject(s)
Humans , Blood Coagulation Tests , Coated Materials, Biocompatible , Chemistry , Heparin , Pharmacology , Materials Testing , Partial Thromboplastin Time , Polyurethanes , Chemistry , Surface PropertiesABSTRACT
Nanotechnology is a newly rising subject.In order to reflect achievements and developments of nanotechnology,applications of nanotechnology to modern medicine are summarized,and its influences were analyzed dialectically.Nanotechnology will evoke a novel technical revolution,although there is still a long way to go before it is widely used.